Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene

BMC Biotechnology

Research

Chuan Yu¹  Zuhua Yu²  Ke Ding²  Chunjie Zhang²  Hairong Wang²  Lei He²  Chengshui Liao²  Cai Zhang² 

[1] Animal Diseases and Public Health Engineering Research Center of Henan Province, Luoyang Polytechnic,  471900, Luoyang, Henan, China;College of Animal Science and Technology/Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Henan University of Science and Technology, 471023, Luoyang, Henan, China;

关键词: Newcastle disease virus;    Reverse genetics system;    Enterotropic;    Red fluorescent protein;   

DOI  :  10.1186/s12896-022-00763-5

 received in 2021-11-05, accepted in 2022-10-20,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundNewcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform.ResultsA full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics.ConclusionThe recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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