Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture

Bioresources and Bioprocessing

Research

Anna Stock¹  Sebastian Gerwald¹  Dirk Weuster-Botz¹  Anna-Lena Thurn¹ 

[1]School of Engineering and Design, Chair of Biochemical Engineering, Technical University of Munich, Boltzmannstr. 15, 85748, Garching, Germany

关键词: Docosahexaenoic acid (DHA);    Eicosapentaenoic acid (EPA);    Microchloropsis salina;    Tisochrysis lutea;    Co-cultivation;    Photoautotrophic microalgae;   

DOI  :  10.1186/s40643-022-00612-5

 received in 2022-08-19, accepted in 2022-11-18,  发布年份 2022

来源: Springer

【 摘 要 】

Marine microalgae have received much attention as a sustainable source of the two health beneficial omega-3-fatty acids docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5). However, photoautotrophic monocultures of microalgae can only produce either DHA or EPA enriched biomass. An alternative may be the photoautotrophic co-cultivation of Tisochrysis lutea as DHA-producer with Microchloropsis salina for simultaneous EPA production to obtain EPA- and DHA-rich microalgae biomass in a nutritionally balanced ratio. Photoautotrophic co-cultivation processes of T. lutea and M. salina were studied, applying scalable and fully controlled lab-scale gas-lift flat-plate photobioreactors with LED illumination for dynamic climate simulation of a repeated sunny summer day in Australia [day–night cycles of incident light (PAR) and temperature]. Monocultures of both marine microalgae were used as reference batch processes. Differences in the autofluorescence of both microalgae enabled the individual measurement, of cell distributions in co-culture, by flow cytometry. The co-cultivation of T. lutea and M. salina in artificial sea water with an inoculation ratio of 1:3 resulted in a balanced biomass production of both microalgae simultaneously with a DHA:EPA ratio of almost 1:1 (26 mgDHA gCDW−1, and 23 mgEPA gCDW−1, respectively) at harvest after depletion of the initially added fertilizer. Surprisingly, more microalgae biomass was produced within 8 days in co-cultivation with an increase in the cell dry weight (CDW) concentration by 31%, compared to the monocultures with the same amount of light and fertilizer. What is more, DHA-content of the microalgae biomass was enhanced by 33% in the co-culture, whereas EPA-content remained unchanged compared to the monocultures.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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