Thrombospondin-2 promotes the proliferation and migration of glioma cells and contributes to the progression of glioma

Chinese Neurosurgical Journal

Research

Yong-Qin Kuang¹  Xin Chen¹  Si-Xun Yu²  Hai-Feng Shu²  Yi-Wen Mei²  Tian-Lan Huang²  Yang Li² 

[1] College of Medicine, Southwest Jiaotong University, No. 111, North Section 1, Second Ring Road, 610031, Chengdu, China;Department of Neurosurgery, General Hospital of Western Theater Command of PLA, No.270 Rongdu Road, Jinniu District, 610083, Chengdu, China;College of Medicine, Southwest Jiaotong University, No. 111, North Section 1, Second Ring Road, 610031, Chengdu, China;

关键词: Glioma;    Migration;    Proliferation;    TSP2;   

DOI  :  10.1186/s41016-022-00308-x

 received in 2022-06-05, accepted in 2022-11-18,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundGliomas, especially high-grade gliomas, are highly malignant with a poor prognosis. Although existing treatments have improved the survival rate of patients with glioma, the recurrence and mortality rates are still not ideal. The molecular mechanisms involved in the occurrence and development of glioma are still poorly understood. We previously reported that thrombospondin-2 (TSP2) expression was increased in tumor specimens from rat models, promoting excitatory synapse formation. However, little is known about the effect of TSP2 on the biological characteristics of glioma.MethodsGlioma and cerebral cortex tissues were collected from 33 patients, and the expression of TSP2 in them was analyzed. Next, the proliferation and migration of TSP2 on glioma cells were analyzed in vitro. At last, a glioma transplantation model was constructed to explore the growth of TSP2 on glioma in vivo.ResultsThe expression of TSP2 in surgical glioma specimens was increased compared to that in the normal cortex. Interestingly, the TSP2 protein level was higher in high-grade glioma (HGG, World Health Organization (WHO) grades 3–4) than in low-grade glioma (LGG, WHO grades 1–2) tissues. Exogenous addition of the TSP2 protein at an appropriate concentration promoted the migration of glioma cells but did not significantly affect their proliferation. Surprisingly, overexpression of TSP2 promoted both the migration and proliferation of cultured glioma cells. Moreover, in vivo experimental data implied that overexpression of TSP2 in C6 cells promoted the malignant growth of gliomas, while knockout of TSP2 slowed glioma growth.ConclusionsTSP2 promotes the migration and proliferation of glioma cells, which may provide new ideas for blocking glioma progression.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

12888_2022_4451_Article_IEq2.gif

12982_2022_119_Article_IEq164.gif

Fig. 1

12982_2022_119_Article_IEq158.gif

12982_2022_119_Article_IEq136.gif

12982_2022_119_Article_IEq133.gif

12888_2022_4365_Article_IEq28.gif

12888_2022_4365_Article_IEq23.gif

12888_2022_4365_Article_IEq11.gif

12888_2022_4365_Article_IEq3.gif

12982_2022_119_Article_IEq224.gif