Nutritional and immunological evaluation of Nannochloropsis oculata as a potential Nile tilapia-aquafeed supplement

BMC Veterinary Research

卷:19

Research Article

Asmaa A. Khaled¹  Samia Elbahnaswy²  Eman Zahran²  Elsayed A. Eldessouki³  Fatma Ahmed⁴  Iman Ibrahim⁵ 

[1] Animal and Fish Production Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt;

[2] Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt;

[3] Department of Fish Health and Diseases, Faculty of Fish Resources, Suez University, Suez, Egypt;

[4] Department of Zoology, Faculty of Science, Sohag University, 82524, Sohag, Egypt;

[5] Pathology Department, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt;

关键词: Fish;    Fatty acids;    Growth;    Histomorphology;    Immunity;    Microalgae;   

DOI  :  10.1186/s12917-023-03618-z

 received in 2022-07-29, accepted in 2023-04-03,  发布年份 2023

来源: Springer

【 摘 要 】

Nannochloropsis oculata (N. oculata) is a marine microalga containing bioactive compounds and a high omega-3 polyunsaturated fatty acid (ω-3 PUFAs). Therefore, it is very promising for nutraceutical and the functional food industry applications. Three groups of Nile tilapia (forty-five fish/group) were fed on basal diets or diets containing 5% (N5) or 10% (N10) of the microalga N. oculata for seven weeks. Fish growth performance, proximate composition, and lipid (fatty acids/ FAs and lipoproteins) profile were estimated. In addition, the expression pattern of some lipid metabolism and immune-relevant genes were assessed. An enhancement in whole body crude protein and growth indices of Nile tilapia was observed on both the supplemented groups N5 and N10. Higher levels of high-density lipoproteins (HDL); and lower levels of the low-density lipoproteins (LDL) were evident in both supplemented groups, while the cholesterol and triglycerides (TG) levels were similar among groups. Ω-3 PUFAs were the significant FAs profile of tilapia fed on N. oculata-supplemented diets in terms of eicosapentaenoic acid, docosahexaenoic acid, and n3/n6 ratio. Concerning the gene expression pattern, heat-shock protein70, glutathione-S-transferase, glutathione peroxidase, and interleukin-1β (IL-1β) were elevated significantly in both supplemented groups. IL-10 is only upregulated in the N10 group. The lipid metabolism-related gene expression showed downregulation of only fatty acid synthase (FAS) in both supplemented groups, with no statistical changes in Peroxisome proliferator-activated receptor alpha (PPARα). Tumor necrosis factor-α (TNF-α), Transforming growth factor-β1 (TGF-β1), and the apoptotic related genes [caspase3 and Proliferating cell nuclear antigen (PCNA)] showed insignificant changes among groups. The histopathological examination of the intestine, liver, and spleen supports our findings and confirms the benefits and safeness of N. oculata dietary inclusion. Collectively, N. oculata is a very promising nutraceutical for improving fish health and sustainability of aquaculture production.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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