Association of 410L, 1016I and 1534C kdr mutations with pyrethroid resistance in Aedes aegypti from Ouagadougou, Burkina Faso, and development of a one-step multiplex PCR method for the simultaneous detection of 1534C and 1016I kdr mutations

Parasites & Vectors

卷:16

Research

Manabu Oté¹  Hirotaka Kanuka¹  Tatsuya Sakurai²  Erisha Saiki²  David Weetman³  Philip J. McCall³  Aboubacar Sombié⁴  Félix Yaméogo⁴  Athanase Badolo⁴  Antoine Sanon⁴  Wendegoudi Mathias Ouédraogo⁵ 

[1] Center for Medical Entomology, The Jikei University School of Medicine, Tokyo, Japan;Department of Tropical Medicine, The Jikei University School of Medicine, Tokyo, Japan;

[2] Center for Medical Entomology, The Jikei University School of Medicine, Tokyo, Japan;Laboratory Animal Facilities, The Jikei University School of Medicine, Tokyo, Japan;

[3] Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, UK;

[4] Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso;

[5] Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso;Programme National de Lutte Contre Les Maladies Tropicales Négligées, Ministère de la Santé, Ouagadougou, Burkina Faso;

关键词: Aedes aegypti;    Pyrethroids;    Resistance;    Malathion;    Multiplex PCR;    Kdr;    Burkina Faso;    Dengue;   

DOI  :  10.1186/s13071-023-05743-y

 received in 2022-08-19, accepted in 2023-03-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundSince 2000, Burkina Faso has experienced regular dengue cases and outbreaks, making dengue an increasingly important health concern for the country. Previous studies in Burkina Faso reported that resistance of Aedes aegypti to pyrethroid insecticides was associated with the F1534C and V1016I kdr mutations. The current study reports high resistance of Ae. aegypti populations to pyrethroid insecticides, likely supported by mutations in the voltage-gated sodium channel, here evidenced by genotyping the kdr SNPs V410L, V1016I and F1534C. We also describe a new multiplex PCR-based diagnostic of F1534C and V1016I kdr SNPs.MethodsLarvae of Ae. aegypti were collected from three health districts of Ouagadougou in 2018. The resistance status of Ae. aegypti to permethrin (15 μg/ml) and deltamethrin (10 μg/ml) was tested using bottles and to malathion (5%) using WHO tube tests. All bioassays used 1-h exposure and mortality recorded 24 h post-exposure. Bioassay results were interpreted according to WHO thresholds for resistance diagnosis. The kdr mutations were screened using AS-PCR and TaqMan methods in exposed and non-exposed Aedes mosquitoes.ResultsFemales from all health districts were resistant to permethrin and deltamethrin (< 20% mortality) but were fully susceptible to 5% malathion. The F1534C and V1016I kdr mutations were successfully detected using a newly developed multiplex PCR in perfect agreement with TaqMan method. The 1534C/1016I/410L haplotype was correlated with permethrin resistance but not with deltamethrin resistance; however, the test power was limited by a low frequency of dead individuals in deltamethrin exposure.ConclusionsResistance to pyrethroid insecticides is associated with kdr mutant haplotypes, while the absence of substantial resistance to malathion suggests that it remains a viable option for dengue vector control in Ouagadougou.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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