Integrated microbiome and metabolome analysis reveals novel urinary microenvironmental signatures in interstitial cystitis/bladder pain syndrome patients

Journal of Translational Medicine

Research

Jintao Hu¹  Kuiqing Li¹  Yousheng Yao¹  Caixia Zhang¹  Wenshuang Li¹  Kaiqun Ma²  Zhenming Zheng³ 

[1]Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China

[2]Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China

[3]Department of Urology, Shantou Central Hospital, Shantou, China

[4]Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China

[5]Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, China

关键词: Interstitial cystitis;    Bladder pain syndrome;    Microbiome;    Metabolomics;    Urine;   

DOI  :  10.1186/s12967-023-04115-5

 received in 2023-01-02, accepted in 2023-04-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe pathogenesis of interstitial cystitis/bladder pain syndrome (IC/BPS) has not been elucidated, but urinary microorganisms and metabolites have been shown to be closely associated with the inflammatory response of IC/BPS. Nevertheless, the exact mechanisms related to this response have not been clarified.Methods16S rRNA sequencing and untargeted metabolomics techniques were used to analyse the urinary microbial and metabolite profiles of 30 IC/BPS patients and 30 healthy controls, and correlation analyses were performed to explore the mechanisms by which they might be involved in the inflammatory response of IC/BPS.ResultsTwenty-eight differential genera, such as Lactobacillus and Sphingomonas, were identified. A total of 44 differential metabolites such as 1,3,7-trimethyluric acid and theophylline were screened. The abundance of Lactobacillus and Escherichia-Shigella was significantly higher in the urine of female IC/BPS patients and healthy controls compared to males, while Bacteroides and Acinetobacter were lower than in males. The results of the Pearson correlation analysis suggested that differential microorganisms may influence the composition of metabolites. The Lactobacillus genus may be a protective bacterium against IC/BPS, whereas Sphingomonas may be a pathogenic factor. The differential metabolite theophylline, as an anti-inflammatory substance, may downregulate the inflammatory response of IC/BPS.ConclusionsThis study revealed microbial and metabolite profiles in the urine of IC/BPS patients versus healthy controls in both males and females. We also found some microorganisms and metabolites closely related to the inflammatory response of IC/BPS, which provided directions for future aetiological and therapeutic research.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 1

13638_2023_2232_Article_IEq9.gif

Fig. 2

Fig. 2

Fig. 5

Fig. 4

40623_2023_1812_Article_IEq29.gif

40507_2023_167_Article_IEq415.gif

40507_2023_167_Article_IEq410.gif

Fig. 2