Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis

BMC Ophthalmology

卷:23

Research

Mengchao Zhu¹  Yitong Xu¹  Haisen Sun¹  Jin Li¹  Jiasheng Liu¹  Qingwen Yang¹  Mengdi Zhang¹  Yaqi Wang¹ 

[1] Department of Cataract, Eye Hospital of Wenzhou Medical University, 270# West Xueyuan Road, 325000, Wenzhou, Zhejiang, China;

关键词: Human lens epithelial cells;    Autophagy;    Single-cell RNA-sequencing;    Transmission electron microscopy;   

DOI  :  10.1186/s12886-023-02910-8

 received in 2023-01-28, accepted in 2023-04-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAutophagy is an important process that maintains the quality of intracellular proteins and organelles. There is extensive evidence that autophagy has an important role in the lens. Human lens epithelial cells (HLECs) play a key role in the internal homeostasis of the lens. HLEC subtypes have been identified, but autophagy-prominent cell clusters among HLECs have not been characterized.PurposeTo explore the existence of autophagy-prominent cell clusters in HLECs.MethodsThree donated lenses (HLECs from two whole lenses and HLECs from one lens without the anterior central 6-mm zone) were used for single-cell RNA sequencing (scRNA-seq). AUCell and AddModuleScore analysis were used to identify potential autophagy-prominent cell clusters. Transmission electron microscopy (TEM) was used to confirm the results.ResultsHigh-quality transcripts from 18,120 cells were acquired by scRNA-seq of the two intact lenses. Unsupervised clustering classified the cells into four clusters. AUCell and AddModuleScore analysis revealed cluster 1 is autophagy-prominent. scRNA-seq analysis of HLECs from the lens capsule lacking the central zone confirmed the cluster 1 HLECs was located in the central capsule zone. The TEM result showed that greater autophagy activity was observed in the HLECs in central capsule zone, which further supported the above conclusions based on scRNA-seq analysis that autophagy was prominent in the central zone where the cluster 1 HLECs located.ConclusionsWe identified an autophagy-prominent cell cluster among HLECs and revealed that it was localized in the central zone of the lens capsule. Our findings will aid investigations of autophagy in HLECs and provide insights to guide related research.

【 授权许可】

CC BY   
© The Author(s) 2023

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