Steroidal saponin profiles and their key genes for synthesis and regulation in Asparagus officinalis L. by joint analysis of metabolomics and transcriptomics

BMC Plant Biology

Research

He Wu¹  Qin Cheng¹  Liangqin Zeng¹  Hao Wen¹  Sylvia E. Brown¹  Chun Lin²  Zhengjie Liu²  Zichao Mao³  Xingyu Li⁴ 

[1]College of Agronomy and Biotechnology, Yunnan Agricultural University (YNAU), 650201, Kunming, Yunnan, China

[2]College of Agronomy and Biotechnology, Yunnan Agricultural University (YNAU), 650201, Kunming, Yunnan, China

[3]Institute of Improvement and Utilization of Characteristic Resource Plants, YNAU, Kunming, China

[4]College of Agronomy and Biotechnology, Yunnan Agricultural University (YNAU), 650201, Kunming, Yunnan, China

[5]Institute of Improvement and Utilization of Characteristic Resource Plants, YNAU, Kunming, China

[6]The Laboratory for Crop Production and Intelligent Agriculture of Yunnan Province, Kunming, China

[7]Institute of Improvement and Utilization of Characteristic Resource Plants, YNAU, Kunming, China

[8]The Laboratory for Crop Production and Intelligent Agriculture of Yunnan Province, Kunming, China

关键词: Asparagus officinalis;    Steroidal saponins biosynthesis;    Transcriptomics;    Metabolomics;   

DOI  :  10.1186/s12870-023-04222-x

 received in 2023-01-10, accepted in 2023-04-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAsparagus officinalis L. is a worldwide cultivated vegetable enrichened in both nutrient and steroidal saponins with multiple pharmacological activities. The upstream biosynthetic pathway of steroidal saponins (USSP) for cholesterol (CHOL) synthesis has been studied, while the downstream pathway of steroidal saponins (DSSP) starting from cholesterol and its regulation in asparagus remains unknown.ResultsMetabolomics, Illumina RNAseq, and PacBio IsoSeq strategies were applied to different organs of both cultivated green and purple asparagus to detect the steroidal metabolite profiles & contents and to screen their key genes for biosynthesis and regulation. The results showed that there is a total of 427 compounds, among which 18 steroids were detected with fluctuated concentrations in roots, spears and flowering twigs of two garden asparagus cultivars. The key genes of DSSP include; steroid-16-hydroxylase (S16H), steroid-22-hydroxylase (S22H) and steroid-22-oxidase-16-hydroxylase (S22O-16H), steroid-26-hydroxylase (S26H), steroid-3-β-glycosyltransferase (S3βGT) and furostanol glycoside 26-O-beta-glucosidases (F26GHs) which were correlated with the contents of major steroidal saponins were screened, and the transcriptional factors (TFs) co-expressing with the resulted from synthetic key genes, including zinc fingers (ZFs), MYBs and WRKYs family genes were also screened.ConclusionsBased on the detected steroidal chemical structures, profiles and contents which correlated to the expressions of screened synthetic and TFs genes, the full steroidal saponin synthetic pathway (SSP) of asparagus, including its key regulation networks was proposed for the first time.

【 授权许可】

CC BY   
© The Author(s) 2023

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