CT-DNA/BSA Binding Studies of Thiosemicarbazone-Derivated Zn(II) Complex

npj Clean Water

article

Asuman UÇAR¹  Mükerrem FINDIK²  Emine AKGEMCݲ 

[1] AĞRI İBRAHİM ÇEÇEN ÜNİVERSİTESİ;NECMETTIN ERBAKAN UNIVERSITY

关键词: DNA;    Thiosemicarbazone;    Zn(II) complex;    Bovine serum albumin binding;   

DOI  :  10.17776/csj.1004338

学科分类：社会科学、人文和艺术（综合）

来源: Springer

【 摘 要 】

Zn(II) complex of 2-hydroxy-5-methoxyacetophenone thiosemicarbazone { Zn(HMAT)2} was synthesized andcharacterized by 1H NMR, UV–Vis and FT-IR spectroscopies. Further, X-ray powder diffraction (XRD) analysis ofZn(HMAT)2 was carried out to point out the complexation. The binding affinities of Zn(HMAT)2 with calf thymusDNA (CT-DNA) have been studied by using fluorescence and absorption titration technics. In addition, bovineserum albumin (BSA) binding studies were recorded by fluorescence and UV–Vis spectroscopy. Zn(HMAT)2 is astrong binders of CT-DNA with binding constant (Kb) 3.65×107 M−1. The binding parameters KSV (for EB), Kq (forBSA) and Kb (for BSA) were determined as 8.2×107 M−1, 1.8×1014 M−1 s−1 and 2×107 M−1 respectively.

【 授权许可】

CC BY