Utilization of Expired Platelet Concentrate for Production of Human Platelet Lysate as a Medium for T47D Cell Propagation

Molecular and Cellular Biomedical Sciences: MCBS

article

Diani Mentari¹  Relita Pebrina²  Diah Nurpratami³ 

[1] Faculty of Biology, Universitas Jenderal Soedirman;Blood Bank Technology Study Program;The Indonesian Red Cross

关键词: cell proliferation;    human platelet lysate;    platelet concentrate;    thrombocyte;    T47D;   

DOI  :  10.21705/mcbs.v6i2.254

来源: Cell and BioPharmaceutical Institute

【 摘 要 】

Background:5 days) can increase the proliferation of T47D cells in vitro.Materials and methods: Expired PC samples with a shelf life of 7 and 11 days were used to produce HPL via freeze/thaw method. pH, total protein content, glucose and albumin levels were measured. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure proliferation rate and doubling time of HPL-treated T47D cells.Results: After HPL production, the glucose level was influenced by the pH (p=0.003), and albumin level was influenced by total protein content (p=0.030). HPL stored for 7 and 11 days increased cell proliferation rate by 1.41 and 1.80 times higher than 10% FBS, respectively. HPL produced from expired PC did not cause morphological abnormality of the cells. In this study, the glucose levels affected cell proliferation (p=0.030). High glucose levels inhibited T47D cell proliferation.Conclusion: Expired PC can be used as a potential material for HPL production, since HPL produced from expired PC increases cell proliferation rate and shortens cell doubling time.

【 授权许可】

Unknown