Cisplatin-induced azoospermia and testicular damage ameliorated by adipose-derived mesenchymal stem cells

Biological Research

article

Ismail, Hamdy Y.¹  Shaker, Nora A.¹  Hussein, Shaymaa²  Tohamy, Adel³  Fathi, Mohamed⁴  Rizk, Hamdy¹  Wally, Y. R.¹ 

[1] Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo University;Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University;Department of Toxicology & Forensic Medicine, Faculty of Veterinary Medicine, Cairo University;Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University

关键词: Testicular degeneration;    Sperm analysis;    Oxidative stress;    Cisplatin side-effects;   

DOI  :  10.1186/s40659-022-00410-5

学科分类：社会科学、人文和艺术（综合）

来源: BioMed Central

【 摘 要 】

The testes are highly susceptible to the adverse effects of chemotherapy and radiation at all stages of life. Exposure to these threats mainly occurs during cancer treatment and as an occupational hazard in radiation centers. The present study investigated the regenerative ability of adipose-derived mesenchymal stem cells (ADMSCs) against the adverse effects of cisplatin on the structure and function of the testes. New Zealand white rabbits (N = 15) were divided into three groups of five: a negative control group (no treatment), a cisplatin group (single dose of cisplatin into each testis followed three days later by a PBS injection), and a cisplatin + ADMSCs group (cisplatin injection followed three days later by an ADMSC injection). On day 45 post-treatment, serum testosterone levels were evaluated, and the testes and epididymis were collected for histology, oxidative stress examination, and epididymal sperm analysis. Cisplatin caused damage to the testicular tissue and decreased serum testosterone levels, epididymal sperm counts, and oxidants. An antioxidant imbalance was detected due to increasing malondialdehyde (MDA) and reduced glutathione (GSH) levels in testicular tissue. The ADMSC-treated group displayed a moderate epididymal sperm count, adequate antioxidant protection, suitable hormone levels, and enhanced testicular tissue morphology. ADMSCs treatment repaired damaged testicular tissue, enhanced biochemical parameters, and modified pathological changes caused by cisplatin.

【 授权许可】

CC BY|CC0