Elevated atmospheric CO 2 concentrations caused a shift of the metabolically active microbiome in vineyard soil

BMC Microbiology

article

Rosado-Porto, David¹  Ratering, Stefan¹  Wohlfahrt, Yvette³  Schneider, Bellinda¹  Glatt, Andrea¹  Schnell, Sylvia¹ 

[1] Institute of Applied Microbiology, Justus Liebig University;Faculty of Basic and Biomedical Sciences, Simón Bolívar University;Department of General and Organic Viticulture, Hochschule Geisenheim University

关键词: Active soil bacterial community;    Carbon cycle;    Nitrogen cycle;    Vineyard;    rRNA;    mRNA quantification;    CO2;    FACE;   

DOI  :  10.1186/s12866-023-02781-5

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Elevated carbon dioxide concentrations (eCO2), one of the main causes of climate change, have several consequences for both vine and cover crops in vineyards and potentially also for the soil microbiome. Hence soil samples were taken from a vineyard free-air CO2 enrichment (VineyardFACE) study in Geisenheim and examined for possible changes in the soil active bacterial composition (cDNA of 16S rRNA) using a metabarcoding approach. Soil samples were taken from the areas between the rows of vines with and without cover cropping from plots exposed to either eCO2 or ambient CO2 (aCO2). Diversity indices and redundancy analysis (RDA) demonstrated that eCO2 changed the active soil bacterial diversity in grapevine soil with cover crops (p-value 0.007). In contrast, the bacterial composition in bare soil was unaffected. In addition, the microbial soil respiration (p-values 0.04—0.003) and the ammonium concentration (p-value 0.003) were significantly different in the samples where cover crops were present and exposed to eCO2. Moreover, under eCO2 conditions, qPCR results showed a significant decrease in 16S rRNA copy numbers and transcripts for enzymes involved in N2 fixation and NO2− reduction were observed using qPCR. Co-occurrence analysis revealed a shift in the number, strength, and patterns of microbial interactions under eCO2 conditions, mainly represented by a reduction in the number of interacting ASVs and the number of interactions. The results of this study demonstrate that eCO2 concentrations changed the active soil bacterial composition, which could have future influence on both soil properties and wine quality.

【 授权许可】

CC BY|CC0