Composition, structure, and functional shifts of prokaryotic communities in response to co-composting of various nitrogenous green feedstocks

BMC Microbiology

article

Matheri, Felix¹  Kambura, Anne Kelly³  Mwangi, Maina²  Ongeso, Nehemiah⁴  Karanja, Edward¹  Adamtey, Noah⁵  Mwangi, Elias Kihara²  Mwangi, Edwin¹  Tanga, Chrysantus¹  Musyoka, Martha Wangu¹  Runo, Steven² 

[1]International Centre for Insect Physiology and Ecology

[2]Kenyatta University

[3]Taita Taveta University

[4]The University of Nairobi

[5]International Division, Research Institute of Organic Agriculture

[6]Biofarms ,(K) Limited

关键词: Microbe-microbe interactions;    Lantana;    Tithonia;    Grass;    Organic farming;    Composting;   

DOI  :  10.1186/s12866-023-02798-w

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Thermophilic composting is a promising method of sanitizing pathogens in manure and a source of agriculturally important thermostable enzymes and microorganisms from organic wastes. Despite the extensive studies on compost prokaryotes, shifts in microbial profiles under the influence of various green materials and composting days are still not well understood, considering the complexity of the green material sources. Here, the effect of regimens of green composting material on the diversity, abundance, and metabolic capacity of prokaryotic communities in a thermophilic compost environment was examined. Total community 16S rRNA was recovered from triplicate compost samples of Lantana-based, Tithonia-based, Grass-based, and mixed (Lantana + Tithonia + Grass)- based at 21, 42, 63, and 84 days of composting. The 16S rRNA was sequenced using the Illumina Miseq platform. Bioinformatics analysis was done using Divisive Amplicon Denoising Algorithm version 2 (DADA2) R version 4.1 and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States version 2 (PICRUSt2) pipelines for community structure and metabolic profiles, respectively. In DADA2, prokaryotic classification was done using the Refseq-ribosomal database project (RDP) and SILVA version 138 databases. Our results showed apparent differences in prokaryotic community structure for total diversity and abundance within the four compost regimens and composting days. The study showed that the most prevalent phyla during composting included Acidobacteriota, Actinobacteriota, Bacteroidota, Chloroflexi, and Proteobacteria. Additionally, there were differences in the overall diversity of metabolic pathways but no significant differences among the various compost treatments on major metabolic pathways like carbohydrate biosynthesis, carbohydrate degradation, and nitrogen biosynthesis. Various sources of green material affect the succession of compost nutrients and prokaryotic communities. The similarity of amounts of nutrients, such as total Nitrogen, at the end of the composting process, despite differences in feedstock material, indicates a significant influence of composting days on the stability of nutrients during composting.

【 授权许可】

CC BY|CC0