Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells

BMC Microbiology

article

Cheong, Heng Choon¹  Cheok, Yi Ying¹  Chan, Yee Teng¹  Tang, Ting Fang¹  Sulaiman, Sofiah²  Looi, Chung Yeng³  Gupta, Rishein⁴  Arulanandam, Bernard⁴  Chang, Li-Yen¹  Wong, Won Fen¹ 

[1]Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya

[2]Department of Obstetrics and Gynecology, Faculty of Medicine, Universiti Malaya

[3]School of Biosciences, Faculty of Health and Medical Sciences, Taylor’s University

[4]South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio

[5]Department of Immunology, Tufts University School of Medicine

关键词: Chlamydia trachomatis;    Pgp3;    Cytokine;    Chemokine;    IL-6;    IL-8;    TNFAIP;    CXCL1;   

DOI  :  10.1186/s12866-023-02802-3

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Genital Chlamydia trachomatis infection is the most common bacterial sexual transmitted disease that causes severe complications including pelvic inflammatory disease, ectopic pregnancy, and infertility in females. The Pgp3 protein encoded by C. trachomatis plasmid has been speculated to be an important player in chlamydial pathogenesis. However, the precise function of this protein is unknown and thus remains to be thoroughly investigated. In this study, we synthesized Pgp3 protein for in vitro stimulation in the Hela cervical carcinoma cells. We showed that Pgp3 induced prominent expression of host inflammatory cytokine genes including interleukin-6 (IL-6), IL-8, tumor necrosis factor alpha-induced protein 3 (TNFAIP3), and chemokine C-X-C motif ligand 1 (CXCL1), implying a possible role of Pgp3 in modulating the inflammatory reaction in the host.

【 授权许可】

CC BY|CC0