Structural differences in the gut microbiome of bats using terrestrial vs. aquatic feeding resources

BMC Microbiology

article

Corduneanu, Alexandra¹  Wu-Chuang, Alejandra³  Maitre, Apolline³  Obregon, Dasiel⁶  Sándor, Attila D.²  Cabezas-Cruz, Alejandro³ 

[1] Department of Animal Breeding and Animal Production, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca;Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine;UMR BIPAR, Laboratoire de Santé Animale, ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort;INRAE, UR 0045 Laboratoire de Recherches Sur Le Développement de L’Elevage;EA 7310, Laboratoire de Virologie, Université de Corse;School of Environmental Sciences, University of Guelph;Department of Parasitology and Zoology, University of Veterinary Medicine;ELKH-ÁTE Climate Change: New Blood-Sucking Parasites and Vector-Borne Pathogens Research Group

关键词: Bats;    Microbiome;    Bacterial community assembly;    Myotis;    Miniopterus;   

DOI  :  10.1186/s12866-023-02836-7

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Bat gut microbiomes are adapted to the specific diets of their hosts. Despite diet variation has been associated with differences in bat microbiome diversity, the influence of diet on microbial community assembly have not been fully elucidated. In the present study, we used available data on bat gut microbiome to characterize the microbial community assembly of five selected bat species (i.e., Miniopterus schreibersii, Myotis capaccinii, Myotis myotis, Myotis pilosus, and Myotis vivesi), using network analysis. These bat species with contrasting habitat and food preferences (i.e., My. capaccinii and My. pilosus can be piscivorous and/or insectivorous; Mi. schreibersii and My. myotis are exclusively insectivorous; while My. vivesi is a marine predator) offer an invaluable opportunity to test the impact of diet on bat gut microbiome assembly. The results showed that My. myotis showed the most complex network, with the highest number of nodes, while My. vivesi has the least complex structured microbiome, with lowest number of nodes in its network. No common nodes were observed in the networks of the five bat species, with My. myotis possessing the highest number of unique nodes. Only three bat species, My. myotis, My. pilosus and My. vivesi, presented a core microbiome and the distribution of local centrality measures of nodes was different in the five networks. Taxa removal followed by measurement of network connectivity revealed that My. myotis had the most robust network, while the network of My. vivesi presented the lowest tolerance to taxa removal. Prediction of metabolic pathways using PICRUSt2 revealed that Mi. schreibersii had significantly higher functional pathway’s richness compared to the other bat species. Most of predicted pathways (82%, total 435) were shared between all bat species, while My. capaccinii, My. myotis and My. vivesi, but no Mi. schreibersii or My. pilosus, showed specific pathways. We concluded that despite similar feeding habits, microbial community assembly can differ between bat species. Other factors beyond diet may play a major role in bat microbial community assembly, with host ecology, sociality and overlap in roosts likely providing additional predictors governing gut microbiome of insectivorous bats.

【 授权许可】

CC BY|CC0