New native Bacillus thuringiensis strains induce high insecticidal action against Culex pipiens pallens larvae and adults

BMC Microbiology

article

Ma, Xinmin¹  Hu, Jianjian¹  Ding, Chengsong¹  Portieles, Roxana¹  Xu, Hongli¹  Gao, Jingyao¹  Du, Lihua¹  Gao, Xiangyou¹  Yue, Qiulin²  Zhao, Lin²  Borrás-Hidalgo, Orlando¹ 

[1] Joint R and D Center of Biotechnology, RETDA, Yotabio-Engineering Co., Ltd;State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Lab of Microbial Engineering, Qilu University of Technology ,(Shandong Academic of Science)

关键词: Bacillus thuringiensis;    Crystals;    Endotoxins;    Profiling;    Cry and cyt genes;   

DOI  :  10.1186/s12866-023-02842-9

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Mosquitoes of many species are key disease vectors, killing millions of people each year. Bacillus thuringiensis-based insecticide formulations are largely recognized as among the most effective, ecologically safe, and long-lasting methods of managing insect pests. New B. thuringiensis strains with high mosquito control effectiveness were isolated, identified, genetically defined, and physiologically characterized. Eight B. thuringiensis strains were identified and shown to carry endotoxin-producing genes. Using a scanning electron microscope, results revealed typical crystal forms of various shapes in B. thuringiensis strains. Fourteen cry and cyt genes were found in the strains examined. Although the genome of the B. thuringiensis A4 strain had twelve cry and cyt genes, not all of them were expressed, and only a few protein profiles were observed. The larvicidal activity of the eight B. thuringiensis strains was found to be positive (LC50: 1.4–28.5 g/ml and LC95: 15.3-130.3 g/ml). Bioassays in a laboratory environment demonstrated that preparations containing B. thuringiensis spores and crystals were particularly active to mosquito larvae and adults. These new findings show that the novel preparation containing B. thuringiensis A4 spores and crystals mixture might be used to control larval and adult mosquitoes in a sustainable and ecologically friendly manner.

【 授权许可】

CC BY|CC0