Assessment of Outer Retinal Remodeling in theHibernating 13-Lined Ground Squirrel

Investigative Ophthalmology & Visual Science

article

Benjamin S. Sajdak¹  Brent A. Bell²  Tylor R. Lewis¹  Gabriel Luna³  Grayson S. Cornwell³  Steven K. Fisher³  Dana K. Merriman⁴  Joseph Carroll¹ 

[1] Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin;Cole Eye Institute/Ophthalmic Research, Cleveland Clinic;Neuroscience Research Institute, University of California Santa Barbara;Biology & Microbiology, University of Wisconsin Oshkosh;Ophthalmology & Visual Sciences, Medical College of Wisconsin;Biophysics, Medical College of Wisconsin

关键词: ground squirrel;    optical coherence tomography;    hibernation;    cone photoreceptors;    choroid;    cornea;   

DOI  :  10.1167/iovs.17-23120

学科分类：社会科学、人文和艺术（综合）

来源: Association for Research in Vision and Ophthalmology

【 摘 要 】

PURPOSE. We examined outer retinal remodeling of the euthermic and torpid cone-dominant13-lined ground squirrel (13-LGS) retina using optical coherence tomography (OCT) imagingand histology.METHODS. Retinas and corneas of living 13-LGSs were imaged during euthermic and torpidphysiological states using OCT. Retinal layer thickness was measured at the visual streak fromregistered and averaged vertical B-scans. Following OCT, some retinas were collectedimmediately for postmortem histologic comparison using light microscopy, immunofluorescence, or transmission electron microscopy.RESULTS. Compared to OCT images from euthermic retinae, OCT images of torpid retinaerevealed significantly thicker inner and outer nuclear layers, as well as increases in thedistances between outer retinal reflectivity bands 1 and 2, and bands 3 and 4. A significantdecrease in the distance between bands 2 and 3 also was seen, alongside significant thinningof the choriocapillaris and choroid. OCT image quality was reduced in torpid eyes, partly dueto significant thickening of the corneal stroma during this state.CONCLUSIONS. The torpid retina of the hibernating 13-LGS undergoes structural changes thatcan be detected by OCT imaging. Comparisons between in vivo OCT and ex vivohistomorphometry may offer insight to the origin of hyperreflective OCT bands within theouter retina of the cone-dominant 13-LGS.

【 授权许可】

CC BY|CC BY-NC-ND