Purification and characterization of lignin peroxidase from white-rot fungi Pleurotus pulmonarius CPG6 and its application in decolorization of synthetic textile dyes

The Journal of General and Applied Microbiology

article

Vu Dinh Giap¹  Hoang Thanh Duc²  Pham Thi Mai Huong³  Do Thi Hanh³  Do Huu Nghi¹  Vu Dinh Duy⁵  Dang Thu Quynh¹ 

[1] Graduate University of Science and Technology, Vietnam Academy of Science and Technology;HaUI Institute of Technology, Hanoi University of Industry;Department of Chemical Technology, Hanoi University of Industry;Institute of Natural Products Chemistry;Vietnam - Russia Tropical Centre

关键词: Decolorization;    lignin peroxidase;    Pleurotus pulmonarius;    textile dyes;   

DOI  :  10.2323/jgam.2022.05.005

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

From the biotechnological point of view, enzymesare powerful tools that help sustain a clean environment in several ways. The enzymatic biodegradationof synthetic dyes is a promising goal since it reducespollution caused by textile dyeing factory wastewater. Lignin peroxidase (EC 1.11.1.14, LiP) has highredox potential; thus, it is great for application invarious industrial fields (e.g., paper- waste treatment and textile dyeing wastewater treatment). Inthe present study, a LiP from an isolated strainPleurotus pulmonarius CPG6 (PpuLiP) was successfully purified with a specific activity of 6.59 U mg-1.The enzyme was purified by using three-step columnchromatography procedures including DEAE, Sephadex G-75, and HiTrapTM Q FF columns with 17.8-fold purity. The enzyme with a molecular weight of40 kDa exhibited enhanced pH stability in the acidicrange. The activity retention was over 75% at a pHof 3.0 for more than 6 hours. Purified PpuLiP wasable to oxidize a variety of substrates including veratryl alcohol, 2,4-DCP, n-propanol, and guaiacol.The effect of metal ions on PpuLiP activity was analyzed. The study will provide a ground to decolorizedyes from various groups of PpuLiP. PurifiedPpuLiP could decolorize 35% Acid blue 25 (AB25),50% Acid red 129 (AB129), 72% Acid blue 62 (NY3),85% Acid blue 113 (AB113), 55% Remazol Brilliantblue R (RBBR), and 100% Reactive red 120 (RR120)for 12 hours. Most of the dyes were decolorized, butthe heat-denatured enzyme used as negative controlobviously did not decolorize the tested dyes. Theseresults indicate that the PpuLiP has potential application in enzyme-based decolorization of syntheticdyes.

【 授权许可】

Unknown