The Journal of General and Applied Microbiology | |
Recovery of photosynthesis after long-term storage in the terrestrial cyanobacterium Nostoc commune | |
article | |
Toshio Sakamoto1  Yang Wei2  Koki Yuasa3  Yoshitaka Nishiyama3  | |
[1] School of Biological Science and Technology, College of Science and Engineering, Kanazawa University;Division of Biological Sciences, Graduate School of Natural Science and Technology;Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University;Present address: Fisheries Technology Institute, Japan Fisheries Research and Education Agency | |
关键词: anhydrobiosis; antioxidant; carotenoid; desiccation tolerance; tocopherol; | |
DOI : 10.2323/jgam.2022.01.003 | |
学科分类:微生物学和免疫学 | |
来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation | |
【 摘 要 】
The terrestrial cyanobacterium Nostoc commune isan anhydrobiotic organism with extreme longevity.Recovery of photosynthesis by rehydration wasexamined using our laboratory stocks of dryN. commune thalli after long-term storage in adesiccated state. In the samples stored at roomtemperature for over 8 years, photosynthetic oxygenevolution was barely detectable, whereas oxygenconsumption was recovered. There was anexceptional case in which photosynthetic oxygenevolution recovered after 8 years of storage at roomtemperature. Both photosynthetic oxygen evolutionand respiratory oxygen consumption were recoveredin dry thalli stored at -20°C for over 15 years.Consistent with the recovery of photosynthetic oxygen evolution, Fv/Fm was detected in the samplesstored at -20°C at levels similar to those of freshlycollected N. commune colonies. Carotenoids, scytonemin and chlorophyll a appeared to be intact inthe dry thalli stored at -20°C, but β-carotene wasnot detected in the samples stored at room temperature. α-Tocopherol was intact in the samples storedat -20°C but was degraded in the samples stored atroom temperature. These results suggest that drythalli of N. commune are capable of sustaining biological activities for a long time, although they aregradually damaged when stored at room temperature.
【 授权许可】
Unknown
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