The precision study of dual energy X-ray absorptiometry for bone mineral density and body composition measurements in female cynomolgus monkeys

Quantitative Imaging in Medicine and Surgery

article

Bin Guo¹  Qijun Cai¹  Jinci Mai¹  Lu Hou¹  Chunyuan Zeng¹  Jiefeng Gan¹  Zhiqiang Tan¹  Yuefeng Li²  Yong Cheng¹  Jingjie Shang¹  Yongjin Tang¹  Xueying Ling¹  Jian Gong¹  Lu Wang¹  Hao Xu¹ 

[1] Department of Nuclear Medicine , The First Affiliated Hospital of Jinan University;Guangdong Landau Biotechnology Co., Ltd.

关键词: Precision;    dual-energy X-ray absorptiometry (DXA);    cynomolgus monkeys;    bone mineral density (BMD);    body composition;   

DOI  :  10.21037/qims-21-799

学科分类：外科医学

来源: AME Publications

【 摘 要 】

Background: Dual-energy X-ray absorptiometry (DXA) is a well-accepted tool for monitoring skeletal and body composition changes in biomedical studies. The nonhuman primate model is suitable for studies exploring the pathogenesis of and novel treatments for osteoporosis. Our objectives are to determine the precision of DXA detection in cynomolgus monkeys and to identify the difference in precision in lumbar bone mineral density (BMD) with various segment selections. Methods: Thirty adult female cynomolgus monkeys underwent duplicate total body DXA scans. Total body bone mineral density (BMDTB) and body composition, including bone mineral content (BMCTB), lean mass (LMTB), and fat mass (FMTB), were analyzed by enCORE software, while lumbar BMD was obtained by manual region-of-interest analysis. The precision was represented as the root-mean-square standard deviation (RMS-SD) and coefficient of variation (RMS-CV%), and least significant changes (LSCs) were reported. Results: The RMS-SD (RMS-CV%) of the repeated DXA analyses for BMDTB, BMCTB, LMTB and FMTB were 0.002 g/cm2 (0.50%), 0.90 g (0.42%), 0.015 kg (0.49%), and 0.031 kg (2.71%), respectively. The regional BMD precision (RMS-CV%) of the lumbar spine with various combinations ranged from 0.70% to 1.09%, The LSCs with 80% statistical confidence (LSC80) ranged from 1.27% to 1.97%, and LSC95 ranged from 1.94% to 3.01%. Conclusions: DXA provided excellent reproducibility in the measurements of BMD and body composition in nonhuman primates. We find DXA reliable for total and regional measurement in skeletal research and the evaluation of osteoporosis treatment with monkeys as animal models.

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