Development and Validation of UV Spectroscopic Method for Estimation of Guaifenesin In Tablet Dosage Form

American Journal of PharmTech Research

article

Ganesh M. Sanap¹  Prajakta S. Shirode¹  Kalpesh V. Sonar¹ 

[1] Department of Pharmaceutical Chemistry, Arunamai college of Pharmacy, North Maharashtra University

关键词: Guaifenesin;    UV Spectrophotometer;    Melting Point;    Assay Method;    Validation;    Accuracy;    Linearity;    Ruggedness;    Precision.;   

DOI  :  10.46624/ajptr.2019.v9.i3.024

学科分类：药学

来源: American Journal Of Pharmtech Research

【 摘 要 】

To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Guaifenesin in tablet dosage form. The drug is freely soluble in analytical grade Methanol. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade Methanol. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity. The UV spectroscopic method was developed for estimation of Guaifenesin in tablet dosage form and also validated as per ICH guidelines. The drug is freely soluble in analytical grade Methanol, moderately soluble in Benzene and soluble in Chloroform, Glycerol. So, the analytical grade Methanol is used as a diluent in method. The melting point of Guaifenesin was found to be 78-79ËšC (uncorrected). It showed absorption maxima 269 nm in analytical grade Methanol. On the basis of absorption spectrum the working concentration was set on 10µg/ml (PPM). The linearity was observed between 6-14 μg/ml (PPM). The results of analysis were validated by recovery studies. The recovery was found to be 98.75, 101 and 99.17% for three levels respectively. The % RSD for precision was found to be 0.97%. A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Guaifenesin in tablet dosage form. The method could be considered for the determination of Guaifenesin in quality control laboratories.

【 授权许可】

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