Evaluation and Comparison of the Antioxidant Activity of Extractable Polyphenols and Non-extractable Polyphenols of Sour Orange Peel

Jundishapur Scientific Medical Journal

article

Rezaei, Zahra¹  Siahpoosh, Amir² 

[1] Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences;Department of Pharmacognosy, Faculty of Pharmacy, Jundishapur University of Medical Sciences

关键词: Sour orange;    Extractable polyphenol;    Non-extractable polyphenol;    ABTS+DPPH;   

DOI  :  10.32598/JSMJ.20.6.2133

学科分类：基础医学

来源: Ahvaz Jundishapur University of Medical Sciences

【 摘 要 】

Background and Objectives Research on food polyphenols has experienced a huge development during thelast century. Most studies on polyphenolic compounds have focused on extractable polyphenols (EPP). However,an important polyphenolic fraction is ignored (Non-Extractable polyphenols (NEPP)) because they aretrapped in the corresponding extraction residues. In this study, the antioxidant EPP and NEPP of sour orangepeel, extracted by aqueous solvent and acid hydrolysis (with two different solvent systems) respectively, areevaluated and compared.Methods Antioxidant activity was evaluated by four tests of radical inhibition of 2, 2-diphenyl-1-picrylhydrazyl(DPPH), 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP)and iron chelating. The amount of polyphenolic compounds, flavonoids, oligomeric proanthocyanidins, andflavanones were respectively determined by folin-ciocalteu, Aluminium chloride (AlCl3) and Ultraviolet–visiblespectroscopy (UV-visible). Also, the anti-lipid peroxidation effect in rat liver was measured.Results The results of antioxidant analysis showed that NEPP with methanol/Sulfuric Acid Solvent had thehighest level of antioxidant activity in sour orange peel. It can be due to the high polyphenolic compounds inthis extraction compared to the others.Conclusion All extracts had antioxidant activity and can be suggested as a potential natural source in food andpharmaceutical industries.

【 授权许可】

Unknown