Acute phase of COVID-19 is associated with elevated plasmablasts in the blood

Bratislava Medical Journal

article

Ondrej BERAN¹  Katerina MATUSKOVA¹  Zofia BARTOVSKA¹  Oldrich BARTOS¹  Michal HOLUB¹ 

[1]Department of Infectious Diseases, First Faculty of Medicine, Charles University

[2]Military University Hospital Prague

[3]Institute of Animal Physiology and Genetics, Laboratory of Fish Genetics, The Czech Academy of Sciences

关键词: COVID-19;    B-lymphocytes;    immunoglobulin A;    immunoglobulin G;   

DOI  :  10.4149/BLL_2022_039

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

OBJECTIVES: The study was aimed at the characterization of humoral immunity in acute SARS-CoV-2 infection. BACKGROUND: Humoral immunity plays a central role in the protection from infection due to SARS-CoV-2, causative agent of coronavirus diseases 2019 (COVID-19). PATIENTS AND METHODS: In 24 adult patients hospitalized with COVID-19, the functional subsets of circulating B-lymphocytes and SARS-CoV-2 specific IgA and IgG antibodies were analyzed using a flow cytometry and immunoassays, respectively. RESULTS: Circulating plasmablasts and memory B-lymphocytes were significantly elevated and regulatory B-lymphocytes significantly decreased in the patients in comparison with 11 age- and sex-matched SARS-CoV-2 seronegative healthy adults. Next, circulating plasmablasts correlated negatively with the levels of SARS-CoV-2 specific IgG antibodies, which were detectable in 9 out of 15 tested patients. In addition, SARS-CoV-2 specific IgA antibodies were detectable in 13 of 15 tested patients and did not demonstrate correlation with any B-lymphocyte subset. CONCLUSION: Severe course of COVID-19 is associated with significant changes of phenotypes of circulating B-lymphocytes and elevated circulating plasmablasts correlate with decreased SARS-CoV-2-specific IgG antibodies (Tab. 2, Fig. 3, Ref. 14).

【 授权许可】

CC BY