Investigation of the nephroprotective effect of dexmedetomidine on colistin-induced nephrotoxicity in rats

Bratislava Medical Journal

article

Ahmet KARATAS¹  Ilker COSKUN²  Erdal BENLI³  Ali ALTINBAS²  Muruvvet AKCAY Celik⁴  Tulin BAYRAK⁵  Ahmet BAYRAK⁵  Ebru CANAKCI² 

[1] Ondokuz Mayis University, Faculty of Medicine, Department of Nephrology;Ordu University, Faculty of Medicine, Department of Anesthesiology and Reanimation;Ordu University, Faculty of Medicine, Department of Urological Surgery;Ordu University, Faculty of Medicine, Department of Medical Pathology;Ordu University, Faculty of Medicine, Department of Medical Biochemistry

关键词: rat;    colistin;    nephrotoxicity;    dexmedetomidine;   

DOI  :  10.4149/BLL_2022_094

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

ACKGROUND: There are very few studies in the literature focusing on whether dexmedetomidine exerts a protective effect on colistin nephrotoxicity. Our study aims to investigate the nephroprotective effect of dexmedetomidine in an experimental model of nephrotoxicity in rats. METHODS: The control group was administered saline (SF) intraperitoneally twice a day. The colistin group received an intraperitoneal (ip) injection of 10 mg/kg of colistin twice a day. The DX10 group received 10 mg/kg of colistin 20 minutes after the intraperitoneal injection of 10 mcg/kg of dexmedetomidine. The DX20 group received 10 mg/kg of colistin 20 minutes after the intraperitoneal injection of 20 mcg/kg of dexmedetomidine. Applications were continued for 7 days, twice a day. All rats were sacrificed on the 8th day after blood and kidney tissue samples were taken. BUN, Creatine, KIM-1 and Endothelin-1 were studied in blood samples. RESULTS: There was a significant difference in the median values of Urea, BUN and Creatine between the groups (p<0.001, p<0.001, p<0.001, respectively). There was a significant difference in the median values of KIM-1 and Endothelin-1 between the groups (p=0.009, p=0.001, respectively). A significant difference was observed between the histopathological scores of the groups (p<0.001). CONCLUSION: Dexmedetomidine significantly decreased the elevated levels of BUN, Creatinine, KIM-1, and Endothelin-1 induced by colistin. Dexmedetomidine, at both doses, histopathologically prevented apoptosis and reduced the number of necrotic cells in the kidneys. Dexmedetomidine provides renoprotective effects, therefore it is a valuable sedation agent for clinicians working in intensive care units.

【 授权许可】

CC BY