Analysis of HLA-G 5′URR regulatory polymorphisms in Slovak women with pre-eclampsia

Bratislava Medical Journal

article

V. Durmanova¹  J. Drobny²  J. Dlhopolcek²  M. Bucova¹ 

[1] Institute of Immunology, Faculty of Medicine, Comenius University in Bratislava;Department of Obstetrics and Gynecology, Faculty of Medicine, University Hospital Bratislava, Comenius University in Bratislava

关键词: HLA-G;    pre-eclampsia;    gene polymorphism;    sequencing;    diagnostics;   

DOI  :  10.4149/BLL_2021_138

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

OBJECTIVES: The objective of this study was to identify the impact of the selected HLA-G gene polymorphisms in the 5′URR region on the risk to develop pre-eclampsia. BACKGROUND: Pre-eclampsia (PE) is a serious multisystem disorder that affects women during pregnancy. Despite many research studies, the pathology of PE is not fully understood. Human leukocyte antigen G (HLA-G) belongs to the molecules that induce immune tolerance at the fetal-maternal interface. HLA-G expression was found to be impaired in the women suffering from PE suggesting its involvement in the development of PE. METHODS: 116 women with pre-eclampsia and 130 women with normotensive pregnancy were included in the study. The 16 gene polymorphisms in the HLA-G 5′URR region (promoter) affecting HLA-G expression were typed by direct sequencing. RESULTS: The -201AA genotypes in recessive model were significantly more frequent in women with pre-eclampsia than in the controls (p = 0.047). Furthermore, the analysis of HLA-G 5′URR variants with clinical findings of PE showed a significant association of the -533delA-/+ genotype with a higher mean level of diastolic blood pressure (p = 0.02). CONCLUSION: Our results suggest a genetic association of selected HLA-G 5′URR variants with a risk of developing pre-eclampsia and possible contribution of HLA-G to disease pathology (Tab. 4, Ref. 51).

【 授权许可】

CC BY