Differential collagen expression in kidney and heart during hypertension

Bratislava Medical Journal

article

S. Stanchev¹  N. Stamenov¹  V. Kirkov¹  E. Dzhambazova²  D. Nikolov³  A. Paloff¹ 

[1] Department of Anatomy, Histology and Embryology, Medical University of Sofi a;Department of Chemistry, Biochemistry, Physiology and Pathophysiology, Faculty of Medicine, Sofi a University ‘St. Kliment Ohridski’;Clinic of Cardiac Surgery, Acibadem City Clinic Tokuda Hospital

关键词: collagen;    kidney;    myocardium;    hypertension;    immunohistochemistry;    spontaneously hypertensive rats (SHR);   

DOI  :  10.4149/BLL_2020_011

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

AIM: The aim of the present study was to investigate the immunohistochemical expression of selected collagen types, namely collagen types I and V and procollagen type III in the renal parenchyma and interstitium and in the myocardium of spontaneously hypertensive rats. MATERIAL AND METHODS: For the present study, we used two age groups of 6- and 12-month-old spontaneously hypertensive rats. An immunohistochemical analysis was conducted with monoclonal antibodies against collagen types I and V and procollagen type III. A semi-quantitative analysis of immunostaining intensity was conducted with the Image J software. RESULTS: In the kidney, all three molecules showed higher expression at the age of 12 months, which was particularly notable for procollagen type III and collagen type V, which stained as highly-positive. In the myocardium, the immunoreactivity of collagen types I and V was stronger in 12-month-old animals, while that of procollagen type III did not change substantially. CONCLUSION: The present study suggests a role of collagen types III and V in hypertensive kidney disease, while also establishing the role of increased expression of collagen types I and V in adverse myocardial remodeling (Tab. 1, Fig. 2, Ref. 48).

【 授权许可】

CC BY