Ribotypes in isolates and testing algorithm of C. difficile infections in the studied sample

Bratislava Medical Journal

article

Z. Stofkova¹  E. Novakova¹  M. Novak² 

[1] Institute of Microbiology and Immunology, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin;Department of Public Health, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin

关键词: toxigenic C;    difficile;    diagnostics;    real-time PCR;    ribotyping;   

DOI  :  10.4149/BLL_2020_027

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

BACKGROUND: Clostridium (Clostridioides) difficile is the most common pathogen of nosocomial and antibiotic-related diarrhea in health-care facilities. The aim of the analysis was to show the testing algorithm and to identify hypervirulent strains (suspected RT 027). METHODS: The retrospective analysis of patient samples suspected on CDI was carried out by a two-step algorithm. Biological specimens were analysed by GDH or culture, immunoenzymatic assay on toxins A/B and selected samples also by a real-time PCR. RESULTS: In 1006 specimen suspected on CDI, 202 specimens were evaluated as positive in the two-step algorithm. Conflicting results (64 C. difficile isolates) were tested in a three-step algorithm by a real-time PCR and revealed 59 toxigenic and non RT 027 ribotypes. Statistically significant dependence among the independent variables, such as: diagnostic parameters and length of hospitalization (p = 0.175) and C. difficile (suspected RT027) ribotypes was not found. CONCLUSION: The results of PCR ribotyping showed a high prevalence of hypervirulent and toxigenic ribotypes in the studied sample. A resistance to vancomycin was found in one isolate. The PCR method contributed to the rapid laboratory diagnosis and thus treatment of high risk patients or was used as a third step in in the case of unclear results of standard diagnostic methods (Tab. 1, Fig. 4, Ref. 18). Text in PDF www.elis.sk.

【 授权许可】

CC BY