Histological, immunohistochemical and biochemical effects of bee bread on stomach tissue of obese rats

Bratislava Medical Journal

article

Z. Doganyigit¹  B. Yakan²  M. Soylu³  E. Kaymak¹  A. Okan¹  S. Silici⁴ 

[1] Yozgat Bozok University, Faculty of Medicine, Department of Histology and Embryology;Erciyes University, Faculty of Medicine, Department of Histology and Embryology;Biruni University, Faculty of Health Sciences, Nutrition and Dietetic Department;Erciyes University, Seyrani Faculty of Agriculture, Department of Medical Biotechnology;Natural Therapy Company

关键词: bee bread;    leptin;    ghrelin;    stomach;    obesity;    rat;   

DOI  :  10.4149/BLL_2020_083

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

Obesity is a major health problem threatening humanity in medical, social and psychological dimensions. In this study, we aimed to determine the histological, immunohistochemical and biochemical effects of bee bread, added to diets of obese rats in different doses, on leptin and ghrelin expression. In the study, 40 female Sprague‒Dawley (200‒250 g) rats were randomly divided into 5 equal groups and then assigned to control and obesity groups. The obesity group consisted of four subgroups: high‑fat diet group, 100 and 200 mg/kg/bw groups, and metformin group. Histopathological evaluation revealed structural deterioration and necrotic areas in the epithelium and glands of the obese rats’ stomach tissue, while in their serum and gastric tissues, the MDA level was significantly higher than in the other groups. There was a negative correlation between leptin and ghrelin levels. Apoptotic cells increased with obesity, but the application of beebread was similarly effective as metformin administration in reducing this increase (Tab. 5, Fig. 4, Ref. 51).

【 授权许可】

CC BY