Recombinant human MMP-2 associated with monoolein improves bone repair

Bratislava Medical Journal

article

F A. T. de Figueiredo¹  J. P. Azevedo²  M. G. Lara³  C. A. Meschiari⁴  J. P. M. Issa²  R. F. Gerlach²  T. C. L. Lamano² 

[1] Department of Dental Materials and Prothesis, School of Dentistry of Ribeirao Preto, University of Sao Paulo – Av. Cafe S/N;Department of Basic and Oral Biology, School of Dentistry of Ribeirao Preto, University of Sao Paulo – Av. Cafe S/N;Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo – Av. Cafe S/N;Department of Physiology, School of Medicine of Ribeirao Preto, University of Sao Paulo – Av. dos Bandeirantes 3900

关键词: bone;    bone defect;    monoolein;    rhMMP-2;    biomaterial;   

DOI  :  10.4149/BLL_2020_095

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

OBJECTIVE: The main objective of the present study was to investigate the possible osteostimulatory action of recombinant human matrix metalloproteinase-2 (rhMMP-2) implanted in a bone defect made in calvaria of rats, bounded to the monoolein as carrier. METHODS: Forty-four adult male Wistar rats (about 600 g body weight) underwent surgery in order to create a spherical defect in parietal bone on the right side of the median sagittal suture by using 4 mm diameter of a trephine drill. Animals were divided into three groups: no treatment (control, C), treatment with rhMMP-2 diluted in monoolein liquid crystal (rhMMP-2) and negative control with monoolein (M). The groups were divided into two experimental times, 2- and 4-weeks of experimental time. RESULTS: The rate of new-formed bone, estimated by the number of points on new-formed cancellous bone, was in enhanced rhMMP-2 group in both periods in comparison to C or M groups. CONCLUSION: There was no difference in bone neoformation between second to fourth week within groups. In the present study, monoolein alone had a negative role in the post-operative surgery, but monoolein associated with +rhMMP-2 had a positive role on releasing rhMMP-2 and enhance the rate of new-formed bone (Tab. 1, Fig. 5, Ref. 71).

【 授权许可】

CC BY