Recombinant adiponectin inhibits inflammation processes via NF-kB pathway in acute pancreatitis

Bratislava Medical Journal

article

K. Dikmen¹  H. Bostanci¹  H. Gobut²  A. Yavuz²  M. Alper³  M. Kerem¹ 

[1] Gazi University, School of Medicine, Department of Genaral Surgery;Yildirim Beyazit University, Yenimahalle Training and Research Hospital, Department of Genaral Surgery;Diskapi Yildirim Bayezit Training and Education Hospital, Department of Pathology

关键词: acute pancreatitis;    recombinant adiponectin;    cytokines;    nuclear factor kappa B;   

DOI  :  10.4149/BLL_2018_110

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

PURPOSE:  Adiponectin is a protein stemming from adipose tissue and having strong anti-inflammatory properties. We aimed to assess the damage diminishing effects of recombinant adiponectin  (rAD) through NF-kB in the experimental acute pancreatitis  (AP) model. MATERIALS AND METHODS:  Acute pancreatitis was created by applying 50 µg/kg dose of intraperitoneal cerulean. The rats were randomised and divided into 3 groups as control, AP and rAD groups. Moreover, the rats in each group were divided into two sub-groups as 24th and 48th hour subgroups. rAD was injected in the study group intraperitoneally. Tissue and blood samples were taken after 24 and 48 hours. Histopathological assessment and NF-kB activity were investigated in pancreatic tissue. RESULTS:  Serum TNF-a, IL-1b and IL-6 levels were found to be statistically significant in the AP group compared to the rAD group in the 24th and 48th hour  (p < 0.05). Similarly, NF-kB activity was also found to be significant in the AP group both in the 24th and 48th hour  (p < 0.05). There were significant differences in the AP and the rAD groups histopathologically in terms of edema, inflammation, vacuolisation and necrosis  (p < 0.001). CONCLUSION:  rAD has significantly reduced NF-kB activity, cytokine levels and tissue damage  (Tab. 1, Fig. 1, Ref. 51).

【 授权许可】

CC BY