Microbiota molecular identification in the daycare unit in the hospital

Journal of applied sciences research

article

Jummanah Sameh Showlag¹  Ahmed M. Al-Hejin¹  Noor Mohammed Bataweel¹  Mohamed Abu-Zaid³ 

[1] Department of Biological Sciences, Faculty of Science, P.O. Box 80203, King Abdulaziz University;King Fahad Medical Research Center, P.O. Box 80216, King Abdulaziz University;Princess Dr. Najla Bint Saud Al-Saud Center for Excellence Research, King Abdulaziz University;Microbial Genetics Department, Genetic Engineering and Biotechnology Division, National Research Center

关键词: Infection;    Microbial;    Molecular;    PCR;    Microbiota;    16s rRNA;    Daycare;   

DOI  :  10.22587/jasr.2021.17.4.1

学科分类：社会科学、人文和艺术（综合）

来源: AENSI

【 摘 要 】

The patient after surgical need a therapeutic period that requires brief-term hospitalization in the daycare unit, and they are usually immunocompromised that can easily have a nosocomial infection. The environment and equipment surround the patient can be a factor of transmission. This study investigates the presence of bacteria by isolate and identifies them by microbial, biochemical, and molecular techniques. 60 swab samples were isolated from the daycare unit in king Abdulaziz university hospital. Samples collected from equipment's surface (bathroom, bed, table, fridge, chair, closet, wall, Iv pump, Sphygmomanometer) and nurses' hands. The results indicated that bacilli and (8) cocci were based on cultural, morphological and biochemical characteristics (60) of bacterial isolates. There were also Gram-positive isolates (67) and Gramnegative isolates (1). Five (5) generic ware isolates are based on 16S rRNA gene sequencing techniques, namely Bacillus, Staphylococcus, Microbacterium, Escherichia coli, and Bhargava. The most occurring bacteria belonged to Bacillus subtilis. Overall, the present study indicated that high touch surfaces are habitually contaminated with pathogens that cased infection to the patient, and it is important to focus the cleaning of these sites and improve the cleaning protocol to controlling the nosocomial infection.

【 授权许可】

Unknown