Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met) polymorphism

Stem cell research

article

Sandra Horschitz¹  Josef Frank³  Philipp Koch¹ 

[1]Hector Institute for Translational Brain Research, Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim

[2]German Cancer Research Center

[3]Department of Genetic Epidemiology in Psychiatry, Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim

DOI  :  10.1016/j.scr.2022.102989

学科分类：生理学

来源: Academic Press

【 摘 要 】

Brain-derived neurotrophic factor (BDNF) has been implicated in a multitude of neurodevelopmental processes including neuronal differentiation, axonal outgrowth, synaptic plasticity, or survival. One human-specific single nucleotide polymorphism (rs6265) in the BDNF gene causes a substitution of valine (Val) to methionine (Met) at codon 66 in the pro domain of the protein (Val66Met). This substitution is associated to reduced hippocampal volumes, poor performance on hippocampal-dependent memory tasks, and some mental disorders such as schizophrenia, depression or Alzheimer’s disease. Here we generated three iPSC lines from healthy donors, either homozygous (Val/Val and Met/Met) or heterozygous (Val/Met) for the polymorphism.

【 授权许可】

CC BY|CC BY-NC-ND