Generation of two induced pluripotent stem cell lines (TMOi001-A-5, TMOi001-A-6) carrying variants in DISC1 exon 2 using CRISPR/Cas9 gene editing

Stem cell research

article

Johanna Heider¹  Denise Sperlich¹  Sabrina Vogel¹  Ricarda Breitmeyer¹  Hansjürgen Volkmer¹ 

[1] Molecular Biology and Neurobiology, NMI Natural and Medical Sciences Institute at the University of Tübingen

关键词: DISC1;    iPSC;    CRISPR/Cas9;   

DOI  :  10.1016/j.scr.2022.102925

学科分类：生理学

来源: Academic Press

【 摘 要 】

DISC1 is a scaffold protein involved in key developmental processes such as neuronal migration, differentiation and neurogenesis. Genetic variants of the DISC1 gene have been linked to neuropsychiatric disorders like schizophrenia, bipolar disorder and major depression. Here, we generated two isogenic iPSC lines carrying mutations in DISC1 exon 2 using CRISPR/Cas9 gene editing. Both lines express pluripotency markers, can be differentiated into the three germ layers and present a normal karyotype. The generated iPSC lines can be used to study the implications of DISC1 mutations in the context of neuropsychiatric diseases in vitro .

【 授权许可】

CC BY|CC BY-NC-ND