Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin

Stem cell research

article

Peiyan Ni¹  Chuqing Zhou¹  Yajing Meng¹  Rui Xue¹  Xia Yang¹  Lei Li¹  Liansheng Zhao¹  Jinxue Wei¹  Rongjun Ni¹  Yingcheng Wang¹  Xiaohong Ma¹  Wanjun Guo¹  Qiang Wang¹  Tao Li² 

[1] The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University;Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine;NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University

关键词: Schizophrenia (SCZ);    Bipolar disorder (BD);    Induced pluripotent stem cells (iPSCs);    Psychiatric disorders;   

DOI  :  10.1016/j.scr.2022.102832

学科分类：生理学

来源: Academic Press

【 摘 要 】

Schizophrenia (SCZ) and bipolar disorder (BD) are debilitating neurodevelopmental disorders with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by three females. An adolescent female was clinically diagnosed as first-episode SCZ. One of her cousins was clinically diagnosed as BD and another one was unaffected control. Induced pluripotent stem cells (iPSCs) were established with reprograming factors Oct4 , Sox2 , Nanog , Lin28 , c-myc , Klf4 , and SV40LT . All lines presented normal karyotype and highly expressed pluripotency markers in vitro . All iPSCs were capable to differentiate into derivatives of three germ layers in vivo .

【 授权许可】

CC BY|CC BY-NC-ND