Generation of an erythroid progenitor-derived iPSC line, VRISGi002-A, from a healthy 27-year-old Vietnamese donor under a feeder-free system

Stem cell research

article

Thi-Hong Nhung Nguyen¹  Thi-Tuyet Trinh Tran¹  Thi-Hoa Luong¹  Xuan-Hung Nguyen¹ 

[1] Center of Applied Sciences, Regenerative Medicine and Advance Technologies ,(CARA), Vinmec Healthcare System;VNU, University of Science;College of Health Sciences, VinUniversity

关键词: Erythroid progenitor;    Vietnamese iPSC line;    Feeder-free;    Sendai virus;   

DOI  :  10.1016/j.scr.2022.102824

学科分类：生理学

来源: Academic Press

【 摘 要 】

We have established the footprint-free Vietnamese human induced pluripotent stem cell (hiPSC) line, VRISGi002-A, from CD71 + CD235a + erythroid progenitor cells (EPCs) of a 27-year-old healthy donor. The EPCs were enriched from isolated peripheral blood and reprogrammed using Sendai viruses which carried the reprogramming factors c-MYC, SOX2, KLF4, and OCT4 under a feeder-free culture system. The established VRISGi002-A cell line expressed typical pluripotency markers, displayed a normal karyotype, and demonstrated the potential to differentiate into the three germ layers. This hiPSC line could serve as a Vietnamese healthy control model for physiological processes and drug screening.

【 授权许可】

CC BY|CC BY-NC-ND