A 3D-printable device allowing fast and reproducible longitudinal preparation of mouse intestines

Animal Models and Experimental Medicine

article

Christine McDonald¹  Jan Claesen³  Beckey DeLucia³  Sergey Samorezov⁵  Megan T. Zangara¹  Rachel L. Markley³  Lucas J. Osborn³  Karlee B. Schultz³ 

[1] Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University;Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic;Department of Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic;Center for Microbiome and Human Health, Lerner Research Institute, Cleveland Clinic;Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic;College of Arts and Sciences, John Carroll University, University Heights

关键词: adenoma;    dissection;    intestines;    mice;    printing;    three-dimensional;    reproducibility of results;   

DOI  :  10.1002/ame2.12228

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Accurate and reproducible analysis of murine small and large intestinal tissue is key for preclinical models involving intestinal pathology. Currently, there is no easily accessible, standardized method that allows researchers of different skill levels to consistently dissect intestines in a time-efficient manner. Here, we describe the design and use of the 3D-printed “Mouse Intestinal Slicing Tool” (MIST), which can be used to longitudinally dissect murine intestines for further analysis. We benchmarked the MIST against a commonly used procedure involving scissors to make a longitudinal cut along the intestines. Use of the MIST halved the time per mouse to prepare the intestines and outperformed alternative methods in smoothness of the cutting edge and overall reproducibility. By sharing the plans for printing the MIST, we hope to contribute a uniformly applicable method for saving time and increasing consistency in studies of the mouse gastrointestinal tract.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND