In-depth examination of hyperproliferative healing in two breeds of Sus scrofa domesticus commonly used for research

Animal Models and Experimental Medicine

article

Colton H. Funkhouser¹  Liam D. Kirkpatrick¹  Robert D. Smith¹  Lauren T. Moffatt¹  Jeffrey W. Shupp¹  Bonnie C. Carney¹ 

[1] Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute;Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center;Department of Surgery, Georgetown University School of Medicine;The Burn Center, Department of Surgery, MedStar Washington Hospital Center

关键词: animal models;    burns;    Fitzpatrick skin type;    hypertrophic scar;    wound healing;   

DOI  :  10.1002/ame2.12188

学科分类：机械工程学

来源: Wiley

【 摘 要 】

Background Wound healing can result in various outcomes, including hypertrophic scar (HTS). Pigs serve as models to study wound healing as their skin shares physiologic similarity with humans. Yorkshire (Yk) and Duroc (Dc) pigs have been used to mimic normal and abnormal wound healing, respectively. The reason behind this differential healing phenotype was explored here. Methods Excisional wounds were made on Dc and Yk pigs and were sampled and imaged for 98 days. PCR arrays were used to determine differential gene expression. Vancouver Scar Scale (VSS) scores were given. Re-epithelialization was analyzed. H&E, Mason's trichrome, and immunostains were used to determine cellularity, collagen content, and blood vessel density, respectively. Results Yk wounds heal to a “port wine” HTS, resembling scarring in Fitzpatrick skin types (FST) I-III. Dc wounds heal to a dyspigmented, non-pliable HTS, resembling scarring in FST IV–VI. Gene expression during wound healing was differentially regulated versus uninjured skin in 40/80 genes, 15 of which differed between breeds. Yk scars had a higher VSS score at all time points. Yk and Dc wounds had equivalent re-epithelialization, collagen disorganization, and blood vessel density. Conclusions Our findings demonstrate that Dc and Yk pigs can produce HTS. Wound creation and healing were consistent among breeds, and differences in gene expression were not sufficient to explain differences in resulting scar phenotype. Both pig breeds should be used in animal models to investigate novel therapeutics to provide insight into a treatment's effectiveness on various skin types.

【 授权许可】

CC BY|CC BY-NC|CC BY-NC-ND