T cell counts and IL-6 concentration in blood of North African COVID-19 patients are two independent prognostic factors for severe disease and death

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

article

Brahim Belaid¹  Lydia Lamara Mahammad¹  Belgacem Mihi²  Sarah Yasmine Rahali¹  Asma Djidjeli¹  Zineb Larab¹  Lilya Berkani¹  Ismahane Berkane¹  Wafa Sayah¹  Fatma Merah¹  Nouzha Zhor Lazli¹  Lylia Kheddouci¹  Ahmed Kadi³  Mourad Ouali⁴  Rachida Khellafi⁵  Dalila Mekideche⁶  Assia Kheliouen³  Soraya Ayoub⁷  Réda Malek Hamidi⁴  Fawzi Derrar⁸  Merzak Gharnaout⁹  Ines Allam¹  Réda Djidjik¹ 

[1]Immunology Department, Béni-Messous Teaching Hospital, University of Algiers

[2]Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital

[3]Pneumology Department A, Béni-Messous Teaching Hospital, University of Algiers

[4]Intensive Care Department, Béni-Messous Teaching Hospital, University of Algiers

[5]Pneumology Department B, Béni-Messous Teaching Hospital, University of Algiers

[6]Pneumology Department C, Béni-Messous Teaching Hospital, University of Algiers

[7]Internal Medicine Department, Béni-Messous, Teaching Hospital, University of Algiers

[8]Virology Department, Institut Pasteur d'Algérie, University of Algiers

[9]Pneumology Department, Rouiba Hospital, University of Algiers

关键词: COVID-19;    cytokines;    flow cytometry;    lymphocytes;    prognosis;    SARS-CoV2;   

DOI  :  10.1002/JLB.4COVA1020-703R

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The immune system plays a crucial role in the response against severe acute respiratory syndrome coronavirus 2 with significant differences among patients. The study investigated the relationships between lymphocyte subsets, cytokines, and disease outcomes in patients with coronavirus disease 2019 (COVID-19). The measurements of peripheral blood lymphocytes subsets and cytokine levels were performed by flow cytometry for 57 COVID-19 patients. Patients were categorized into two groups according to the severity of the disease (nonsevere vs. severe). Total lymphocytes, T cells, CD4+ T cells, CD8+ T cells, B cells, and natural killer cells were decreased in COVID-19 patients and statistical differences were found among different severity of illness and survival states ( P ˂ 0.01). The levels of IL-6 and IL-10 were significantly higher in severe and death groups and negatively correlated with lymphocyte subsets counts. The percentages of Th17 in the peripheral blood of patients were higher than those of healthy controls whereas the percentages of Th2 were lower. For the severe cases, the area under receiver operating characteristic (ROC) curve of IL-6 was the largest among all the immune parameters (0.964; 95% confidence interval: 0.927–1.000, P   106.44 pg/ml and CD8+ T cell counts <150 cells/μl were found to be associated with mortality. Measuring the immune parameters and defining a risk threshold can segregate patients who develop a severe disease from those with a mild pathology. The identification of these parameters may help clinicians to predict the outcome of the patients with high risk of unfavorable progress of the disease.

【 授权许可】

CC BY