Fluorescence CLEM in biology: historic developments and current super-resolution applications

FEBS Letters

article

Koen van den Dries¹  Jack Fransen¹  Alessandra Cambi¹ 

[1] Department of Cell Biology, Radboud Institute for Molecular Life Sciences;Microscopic Imaging Center, Radboud Institute for Molecular Life Sciences

关键词: cell biology;    correlative microscopy;    electron microscopy;    fluorescence;    fluorescent proteins;    light microscopy;    super-resolution microscopy;   

DOI  :  10.1002/1873-3468.14421

来源: John Wiley & Sons Ltd.

【 摘 要 】

Correlative light and electron microscopy (CLEM) is a powerful imaging approach that allows the direct correlation of information obtained on a light and an electron microscope. There is a growing interest in the application of CLEM in biology, mainly attributable to technical advances in field of fluorescence microscopy in the past two decades. In this review, we summarize the important developments in CLEM for biological applications, focusing on the combination of fluorescence microscopy and electron microscopy. We first provide a brief overview of the early days of fluorescence CLEM usage starting with the initial rise in the late 1970s and the subsequent optimization of CLEM workflows during the following two decades. Next, we describe how the engineering of fluorescent proteins and the development of super-resolution fluorescence microscopy have significantly renewed the interest in CLEM resulting in the present application of fluorescence CLEM in many different areas of cellular and molecular biology. Lastly, we present the promises and challenges for the future of fluorescence CLEM discussing novel workflows, probe development and quantification possibilities.

【 授权许可】

Unknown