期刊论文详细信息
FEBS Letters
The TKFC Ala185Thr variant, reported as ‘null’ for fructose metabolism, is fully active as triokinase
article
João M. Ribeiro1  María Jesús Costas1  Alicia Cabezas1  Brigitte Meunier2  Alexandros Onoufriadis3  José Carlos Cameselle1 
[1] Grupo de Enzimología, Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina y Ciencias de la Salud, Universidad de Extremadura;Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell;St John's Institute of Dermatology, School of Basic and Medical Biosciences, King's College London
关键词: fructose metabolism;    fructose oxidation;    glyceraldehyde crossroads;    glyceraldehyde phosphorylation;    lipogenesis;    rs2260655;    single nucleotide polymorphism;    TKFC;    triokinase and FMN cyclase;   
DOI  :  10.1002/1873-3468.14309
来源: John Wiley & Sons Ltd.
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【 摘 要 】

TKFC -encoded triokinase catalyses glyceraldehyde phosphorylation in fructose metabolism and favours lipogenesis in mice. In Tkfc knockouts or knockdowns, fructose oxidation predominates over lipogenesis. The highly prevalent human variant Ala185Thr-Triokinase/FMN cyclase (TKFC) has been reported to be ‘null’ for fructose metabolism, since Ala185-TKFC rescues the mouse TKFC-deficient phenotype, whereas Ala185Thr-TKFC does not. Such report implies that most humans would display a noncanonical fructose metabolism, but it ignores the well-characterized triokinase activity of Ala185Thr-TKFC. Here, earlier evidence is summarized, along with new evidence that both human variants are equally active in yeast. Therefore, future research on triokinase in the context of human fructose metabolism should consider that Ala185Thr-TKFC is not biochemically ‘null’.

【 授权许可】

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