Engineering ergothioneine production in Yarrowia lipolytica

FEBS Letters

article

Douglas B. Kell¹  Irina Borodina¹  Steven A. van der Hoek¹  Matej Rusnák¹  Irene Hjorth Jacobsen³  José L. Martínez³ 

[1] The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark;Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool;Department of Biotechnology and Biomedicine, Technical University of Denmark

关键词: ergothioneine;    metabolic engineering;    nutraceutical;    phosphate-limitation;    Yarrowia lipolytica;   

DOI  :  10.1002/1873-3468.14239

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ergothioneine is a naturally occurring antioxidant that has shown potential in ameliorating neurodegenerative and cardiovascular diseases. In this study, we investigated the potential of the Crabtree-negative, oleaginous yeast Yarrowia lipolytica as an alternative host for ergothioneine production. We expressed the biosynthetic enzymes EGT1 from Neurospora crassa and EGT2 from Claviceps purpurea to obtain 158 mg·L −1 of ergothioneine in small-scale cultivation, with an additional copy of each gene improving the titer to 205 mg·L −1 . The effect of phosphate limitation on ergothioneine production was studied, and finally, a phosphate-limited fed-batch fermentation in 1 L bioreactors yielded 1.63 ± 0.04 g·L −1 ergothioneine in 220 h, corresponding to an overall volumetric productivity of 7.41 mg·L −1 ·h −1 , showing that Y .  lipolytica is a promising host for ergothioneine production.

【 授权许可】

Unknown