Reactivity of γ-glutamyl-cysteine with intracellular and extracellular glutathione metabolic enzymes

FEBS Letters

article

Misa Muraoka¹  Saki Yoshida¹  Moeka Ohno¹  Hideyuki Matsuura¹  Kazuya Nagano¹  Yoshihiko Hirata²  Masayoshi Arai³  Kazumasa Hirata¹ 

[1] Applied Environmental Biology Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University;Biochemical Laboratory, Saraya Co. Ltd.;Natural Products for Drug Discovery Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University

关键词: antioxidant defence;    gamma-glutamyl-cysteine;    glutathione;    glutathione metabolic enzyme;   

DOI  :  10.1002/1873-3468.14261

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gamma-glutamyl-cysteine (γ-EC) is a precursor of glutathione (GSH) biosynthesis. We investigated whether it functions as a substrate for three intracellular and one extracellular GSH metabolic enzymes, which mediate the antioxidant defence function of GSH. Among them, glutathione peroxidase, glutathione S -transferase and γ-glutamyl transferase (GGT) exhibited substrate specificity for γ-EC, whereas glutathione reductase did not. The specificities of γ-EC and its disulphide form to GGT were comparable to GSH and its oxidized form, GSSG respectively. These results indicate that they can supply GSH constituent amino acids, glutamate, cysteine and cystine through degradation by GGT. γ-EC may contribute valuable antioxidant defence properties as a food and cosmetic additive.

【 授权许可】

Unknown