期刊论文详细信息
FEBS Letters
Selective increment of phosphatidylserine on the autophagic body membrane in the yeast vacuole
article
Yuki Yamakuchi1  Yuna Kurokawa1  Rikako Konishi1  Kayoko Fukuda1  Tatsunori Masatani2  Akikazu Fujita1 
[1] Department of Molecular and Cell Biology and Biochemistry, Basic Veterinary Science, Faculty of Veterinary Medicine, Kagoshima University;Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University
关键词: autophagy;    electron microscopy;    freeze fracture;    lipids;    nanoscale;    phosphatidylcholine;    phosphatidylserine;    phosphoinositide;   
DOI  :  10.1002/1873-3468.14167
来源: John Wiley & Sons Ltd.
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【 摘 要 】

In yeast cells, the autophagosome is a double-membrane structure; the inner membrane becomes the autophagic body membrane in the vacuole. Vacuolar enzymes degrade the autophagic body. There is no critical information regarding its selective degradation. Using the electron microscopy method, distributions of four phospholipids were examined in the autophagosomal and autophagic body membranes upon autophagy induction. The labeling of phosphatidylserine (PtdSer) in the autophagic body membrane dramatically increased after it converted from the autophagosome, but remained low in the vacuolar membrane. PtdSer in the autophagic body membrane also increased in atg15 ∆ yeast. These results suggest that the selective increment of PtdSer in the autophagic body, but not the vacuolar, membrane, can explain the selective degradation of the autophagic membrane.

【 授权许可】

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