MXD/MIZ1 transcription regulatory complexes activate the expression of MYC-repressed genes

FEBS Letters

article

Anton Shostak¹  Géza Schermann¹  Axel Diernfellner¹  Michael Brunner¹ 

[1] Heidelberg University Biochemistry Center

关键词: MIZ1;    MNT;    MXD;    MYC;    ZBTB17;   

DOI  :  10.1002/1873-3468.14097

来源: John Wiley & Sons Ltd.

【 摘 要 】

MXDs are transcription repressors that antagonize MYC-mediated gene activation. MYC, when associated with MIZ1, acts also as a repressor of a subset of genes, including p15 and p21 . A role for MXDs in regulation of MYC-repressed genes is not known. We report that MXDs activate transcription of p15 and p21 in U2OS cells. This activation required DNA binding by MXDs and their interaction with MIZ1. MXD mutants deficient in MIZ1 binding interacted with the MYC-binding partner MAX and were active as repressors of MYC-activated genes but failed to activate MYC-repressed genes. Mutant MXDs with reduced DNA-binding affinity interacted with MAX and MIZ1 but neither repressed nor activated transcription. Our data show that MXDs and MYC have a reciprocally antagonistic potential to regulate transcription of target genes.

【 授权许可】

Unknown