The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

FEBS Letters

article

Olanrewaju Raji¹  Jenny Arnling Bååth²  Thu V. Vuong¹  Johan Larsbrink²  Lisbeth Olsson²  Emma R. Master¹ 

[1] Department of Chemical Engineering and Applied Science, University of Toronto;Department of Biology and Biological Engineering, Wallenberg Wood Science Center, Chalmers University of Technology;Department of Bioproducts and Biosystems, Aalto University

关键词: 4-O-methyl d-glucuronic acid;    carbohydrate-active enzymes;    enzyme adsorption;    glucuronoxylan;    hemicellulases;    lignin–carbohydrate complexes;   

DOI  :  10.1002/1873-3468.14019

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase ( Tt CE15A) and two α-glucuronidases ( Sde Agu115A and Axy Agu115A). When supplementing α-glucuronidase with equimolar quantities of Tt CE15A, total MeGlc p A released after 72 h by Sde Agu115A and Axy Agu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined Tt CE15A and Axy Agu115A activities, ~ 34% of MeGlc p A in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble Tt CE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.

【 授权许可】

Unknown