TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility

Reproductive medicine and biology

article

Yuki Oyama¹  Haruhiko Miyata²  Keisuke Shimada²  Tamara Larasati²  Yoshitaka Fujihara²  Masahito Ikawa¹ 

[1]Graduate School of Pharmaceutical Sciences, Osaka University

[2]Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University

[3]Graduate School of Medicine, Osaka University

[4]Department of Bioscience and Genetics, National Cerebral and Cardiovascular Center

[5]The Institute of Medical Science, The University of Tokyo

[6]Center for Infectious Disease Education and Research, Osaka University

关键词: flagellum;    outer dense fiber;    sperm motility;    spermiogenesis;    tubby-like protein 2;   

DOI  :  10.1002/rmb2.12467

学科分类：工业工程学

来源: Wiley

【 摘 要 】

Purpose Tulp2 (tubby-like protein 2) is a member of the tubby protein family and expressed predominantly in mouse testis. Recently, it was reported that Tulp2 knockout (KO) mice exhibited disrupted sperm tail morphology; however, it remains to be determined how TULP2 deletion causes abnormal tail formation. Methods The authors analyzed male fertility, sperm morphology, and motility of two Tulp2 KO mouse lines that were generated using the conventional method that utilizes homologous recombination in embryonic stem (ES) cells as well as the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system. Furthermore, the authors observed the spermatogenesis of Tulp2 KO mice in more detail using scanning and transmission electron microscopy (SEM and TEM). Results Both mouse lines of Tulp2 KO exhibited male infertility, abnormal tail morphology, and impaired sperm motility. No overt abnormalities were found in the formation of the mitochondrial sheath in Tulp2 KO mice using the freeze-fracture method with SEM. In contrast, abnormal outer dense fiber (ODF) structure was observed in Tulp2 KO testis with TEM. Conclusions TULP2 may play roles in the correct formation and/or maintenance of ODF, which may lead to abnormal tail morphology, impaired sperm motility, and male infertility.

【 授权许可】

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