A Genetic Toolkit for Simultaneous Generation of LexA - and QF -Expressing Clones in Selected Cell Types in Drosophila

Journal of Experimental Neuroscience

article

Kota Banzai¹  Ping Shen¹  Daichi Kamiyama¹ 

[1] Department of Cellular Biology, University of Georgia

关键词: Drosophila;    mosaicism;    gene expression;    dendrites;    motor neuron;   

DOI  :  10.1177/26331055211069939

学科分类：药学、药理学、毒理学（综合）

来源: Sage Journals

【 摘 要 】

Visualization and manipulation of defined motoneurons have provided significant insights into how motor circuits are assembled in Drosophila . A conventional approach for molecular and cellular analyses of subsets of motoneurons involves the expression of a wide range of UAS transgenes using available GAL4 drivers (eg, eve promoter-fused GAL4 ). However, a more powerful toolkit could be one that enables a single-cell characterization of interactions between neurites from neurons of interest. Here we show the development of a UAS > LexA > QF expression system to generate randomly selected neurons expressing one of the 2 binary expression systems. As a demonstration, we apply it to visualize dendrite-dendrite interactions by genetically labeling eve + neurons with distinct fluorescent reporters.

【 授权许可】

CC BY|CC BY-NC