Gender-specific reference intervals for serum prolactin concentrations before and after precipitation by polyethylene glycol

Proceedings of Singapore Healthcare

article

Pallavi Chincholkar¹  Chin Pin Yeo¹  Clement Kam Man Ho² 

[1] Department of Clinical Pathology, Singapore General Hospital;Duke-NUS Medical School;Department of Pathology and Laboratory Medicine, KK Women and Children Hospital;Department of Pathology, Sengkang General Hospital

关键词: Macroprolactin;    prolactin;    polyethylene glycol;   

DOI  :  10.1177/20101058211049838

学科分类：社会科学、人文和艺术（综合）

来源: Sage Journals

【 摘 要 】

Prolactin, a hormone secreted by the anterior pituitary, exists in three major forms in circulation. The macroprolactin form is biologically inactive but contributes to elevated serum prolactin concentrations. Precipitation of macroprolactin by polyethylene glycol (PEG) is widely used in clinical laboratories for the screening of macroprolactin. The aim of this study was two-fold. Firstly, we sought to establish locally relevant reference intervals for serum prolactin and post-PEG precipitation prolactin concentrations in both genders on the Roche Cobas Prolactin II electrochemiluminescence immunoassay. Secondly, the prolactin concentrations after precipitation by PEG dissolved in phosphate-buffered saline (post-PEG(PBS)) were compared with those after precipitation by PEG dissolved in deionised water (PEG(diH2O)). Prolactin concentrations were measured by using the Prolactin Gen II assay on a Roche Cobas e601 analyser. Recoveries of prolactin after precipitation by PEG in PBS and PEG in diH2O were 64.7%–115.8% and 60.9–100.8%, respectively. Post-PEG (PBS) prolactin concentrations were not statistically different from post-PEG (diH2O) prolactin concentrations in either gender.

【 授权许可】

CC BY|CC BY-NC