Reporter Systems for Assessments of Extracellular Vesicle Transfer

Frontiers in Cardiovascular Medicine

article

Chaoshan Han¹  Gangjian Qin¹ 

[1] Department of Biomedical Engineering, School of Medicine, University of Alabama at Birmingham;Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham

关键词: exosomes;    cell–cell communication;    reporter;    EVs visualization;    recipient cells;    extracellular vesicle;   

DOI  :  10.3389/fcvm.2022.922420

学科分类：地球科学(综合)

来源: Frontiers

【 摘 要 】

Extracellular vesicles (EVs) are lipid bilayer particles naturally released from most if not all cell types to mediate inter-cellular exchange of bioactive molecules. Mounting evidence suggest their important role in diverse pathophysiological processes in the development, growth, homeostasis, and disease. Thus, sensitive and reliable assessments of functional EV cargo transfer from donor to acceptor cells are extremely important. Here, we summarize the methods EV are labeled and their functional transfer in acceptor cells are evaluated by various reporter systems.

【 授权许可】

CC BY