The Severity of Acute Kidney and Lung Injuries Induced by Cecal Ligation and Puncture Is Attenuated by Menthol: Role of Proliferating Cell Nuclear Antigen and Apoptotic Markers

Frontiers in Medicine

article

Aliaa Anter¹  Al-Shaimaa F. Ahmed¹  Asmaa S. A. Hammad¹  Waleed Hassan Almalki²  Sara Mohamed Naguib Abdel Hafez³  AlShaimaa W. Kasem⁴  Mohamed A. El-Moselhy¹  Mohammad W. Alrabia⁶  Ahmed R. N. Ibrahim⁷  Mahmoud El-Daly¹ 

[1]Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University

[2]Department of Pharmacology and Toxicology, Umm Al-Qura University

[3]Department of Histology and Cell Biology, Faculty of Medicine, Minia University

[4]Department of Pathology, Faculty of Medicine, Minia University

[5]Department of Clinical Pharmacy and Pharmacology, Ibn Sina National College for Medical Studies

[6]Department of Microbiology and Medical Parasitology, Faculty of Medicine, King Abdulaziz University

[7]Department of Clinical Pharmacy, College of Pharmacy, King Khalid University

[8]Department of Biochemistry, Faculty of Pharmacy, Minia University

关键词: menthol;    cecal ligation and puncture;    AKI;    ALI;    PCNA;   

DOI  :  10.3389/fmed.2022.904286

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Objective Sepsis-induced acute lung injury (ALI) and acute kidney injury (AKI) are major causes of mortality. Menthol is a natural compound that has anti-inflammatory and antioxidative actions. Since exaggerated inflammatory and oxidative stress are characteristics of sepsis, the aim of this study was to evaluate the effect of menthol against sepsis-induced mortality, ALI, and AKI. Methods The cecal ligation and puncture (CLP) procedure was employed as a model of sepsis. Rats were grouped into sham, sham-Menthol, CLP, and CLP-Menthol (100 mg/kg, p.o). Key Findings A survival study showed that menthol enhanced the survival after sepsis from 0% in septic group to 30%. Septic rats developed histological evidence of ALI and AKI. Menthol markedly suppressed sepsis induced elevation of tissue TNF-a, ameliorated sepsis-induced cleavage of caspase-3 and restored the antiapoptotic marker Bcl2. Significance We introduced a role of the proliferating cell nuclear antigen (PCNA) in these tissues with a possible link to the damage induced by sepsis. PCNA level was markedly reduced in septic animals and menthol ameliorated this effect. Our data provide novel evidence that menthol protects against organ damage and decreases mortality in experimental sepsis.

【 授权许可】

CC BY